RealTime ready Cell Lysis Kit   實時準備細胞 裂解試劑盒

Reagent for lysing cells prior to two-step real-time RT-PCR applications.

用于兩步實時RT-PCR應(yīng)用前裂解細胞的試劑。

For life science research only. Not for use in diagnostic procedures.

僅供生命科學研究使用。不用于診斷程序。

Overview概述：

Increase convenience:

Replace cumbersome multi-step RNA purification protocols with a simple one-step cell lysis procedure.

增加便利性：

用簡單的一步細胞裂解程序取代繁瑣的多步 RNA 純化程序。

Improve time to result:

Lyse samples in just 5 minutes at room temperature without further steps.

縮短獲得結(jié)果的時間：

在室溫下僅需 5 分鐘即可裂解樣品，無需進一步操作。

Reduce intermediate steps:

Obtain faster, high-quality results by performing DNase treatment during cDNA synthesis.

減少中間步驟：

通過在 cDNA 合成過程中進行 DNase 處理，獲得更快、高質(zhì)量的結(jié)果。

Benefit from broad applicability:

Scale up from 3 to 30,000 cultured cells for real-time PCR applications in 96- to 1536-well formats with or without automation.

受益于廣泛的適用性：

在 96 至 1536 孔格式的實時 PCR 應(yīng)用中，無論是否采用自動化，都可以將 3 到 30,000 個培養(yǎng)細胞放大。

Contents 內(nèi)容

1. Ready-to-use Lysis Buffer (20 mL)

   即用型裂解緩沖液 (20 mL)

• Protector RNase Inhibitor (250 µL - 40 U/µL)

   保護器核糖核酸酶抑制劑 (250 µL - 40 U/µL) 

• Thermolabile Nuclease (250 µL)

   耐熱核酸酶 (250 µL)

Application應(yīng)用：

The RealTime ready Cell Lysis Kit is designed for rapid, high-performance gene expression studies using cultured cells in two-step, real-time RT-PCR applications. The kit uses an advanced one-step protocol for lysing 3 to 30,000 cells within 5 minutes at room temperature. The resulting lysate can be used directly in cDNA synthesis, eliminating the time-consuming RNA purification step. Finally, the cDNA can be analyzed in real-time PCR experiments at any throughput (e.g., LightCycler® 480 Instrument, 96- and 384-well; LightCycler® 1536 Instrument, 1536-well). Due to the user-friendly packaging and easy-to-use protocol, the RealTime ready Cell Lysis Kit can be easily adapted to all experimental and throughput requirements. In addition, the kit easily integrates into all manual and automated cell-based gene expression workflows.

實時準備細胞裂解試劑盒設(shè)計用于在兩步實時 RT-PCR 應(yīng)用中使用培養(yǎng)細胞進行快速、高性能的基因表達研究。該試劑盒采用*一步式方案，可在室溫下 5 分鐘內(nèi)裂解 3 至 30,000 個細胞。所得裂解物可直接用于 cDNA 合成，省去了耗時的 RNA 純化步驟。最后，可以在任何通量的實時 PCR 實驗中分析 cDNA（例如，  LightCycler ®  480 儀器，96 孔和 384 孔；LightCycler ® 1536 儀器，1536 孔）。由于用戶友好的包裝和易于使用的協(xié)議，RealTime ready Cell Lysis Kit 可以輕松適應(yīng)所有實驗和通量要求。此外，該試劑盒可輕松集成到所有手動和自動基于細胞的基因表達工作流程中。